Contact an Account ManagerIsocratic separation of PTH amino acids by HPLC
Summary
Type: HPLC
Application #: 107740
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 254nm
Conditions: eluent: 32% acetonitrile - tetrahydrofuran (9:1) + 68% tetrahydrofuran - water (50:950) containing 0.3 ml/l acetic acid, adjusted to pH 5.15 with 1N NaOH
flow rate 1.5ml/min
temperature 60°C
pressure 2500psi
Authors: Kim SM
Source: Chemie Grünenthal Stolberg Germany
Details
Legend: Separation of PTH amino acids (~ 0.5nmol)
1. aspartic acid
2. carboxymethyl cysteine
3. glutamic acid
4. asparagine
5. glutamine
6. serine
7. threonine
8. glycine
9. histidine
10. arginine